Texas red-phalloidin
WebJun 1, 2024 · Texas Red®-X phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Texas Red®-X dye. Selectively stains F-actin Outstanding fluorescence performance Excitation/Emission: 591/608 nm Superior to antibody staining Optimal for fixed and permeabilized samples In histology freezer ‹ … WebExamine the fluorescence emission intensity from a culture of human lung cells ( MRC-5 line) stained with wheat germ agglutinin conjugated to Texas Red, Alexa Fluor 488 …
Texas red-phalloidin
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WebTexas Red™-X phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Texas Red™-X dye. • Selectively stains F-actin • Outstanding … Texas Red™-X phalloidin is a high-affinity F-actin probe conjugated to our bright, … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebPhalloidin, Alexa Fluor ™ Plus 647 Phalloidin, and Alexa Fluor Plus 750 Phalloidin. Note: For a complete list of fluorescent or biotinylated phalloidin conjugates, see Table 1 on page 5. Contents and storage Contents Cat. No. Amount[1] Storage[2] Fluorescent phalloidin See Table 1 on page 5. 1 vial of lyophilized solids (300 units/assay[3])
WebOct 2, 2024 · F-actin was visualized by Texas Red-phalloidin. Data are representative images of three independent experiments (a, c, and e), and >25 cells were analyzed in three independent experiments (b, d, and f). (g and h) Activities of CDC42, RAC1, and RAC2. Activities of CDC42, RAC1, and RAC2 were measured by GST-PBD pulldown coupled with … WebThe three stains used for this BPAE cell were the Texas Red- Phalloidin (which made actin visible), the BODIPY FL stain (which made tubulin visible), and finally DAPI (which made the nucleus visible). The three excitation wavelengths used to examine the cells were UV (365 nm), yellow/greer (552 nm), and blue (495 nm).
WebMay 20, 2024 · Actin (red) was stained with Texas Red-phalloidin, and nuclei (blue) stained with DAPI. Cells were imaged at magnification ×63, and shown are projections of z-stacks generated with Volocity. D) Volume and E) Percent co-localization of Ltp1 or Php1 in the nuclear region of the images in (C) * p< 0.05, **** p < 0.001 compared to NI. WebThe green stain is beta-galactosidase (ab134435); the red stain is Texas-red-phalloidin, staining filamentous actin; the blue staining is DAPI (4',6-diamidino-2-phenylindole), a DNA-binding dye.Secondary antibodies were fluorescein-labeled affinity-purified goat anti-chicken IgY (1/2000 dilution).
WebTexas Red Operates as a conjugated fluorophore Phalloidin (toxin derived from mushrooms) - binds to actin filaments in the cell Not fluorescent Rhodamine binds to the phalloidin and operates as a rhodamine-phalloidin complex FIX Solution FIX solution contains paraformaldehyde
WebThe actin cytoskeleton was stained with Texas Red ®-X phalloidin (Molecular Probes). To that end, the commercial solution was prepared in PBS and left in dark conditions for 20 min at room temperature (RT). Hoechst (Invitrogen, Molecular Probes) was employed as a contrast marker, to detect cell nuclei. Fully-dense and porous substrates were ... cory gritterWebRhodamine Red-X 560 580 orange Tamara 565 580 red Cy3.5 581 581 596 red Rox 575 602 red Alexa fluor 568 578 603 red Red 613 480;565 613 red Texas Red 615 615 red Alexa fluor 594 590 617 red Alexa fluor 633 621 639 red Allophycocyanin 650 660 red Alexa fluor 633 650 668 red Cy5 650 670 red Alexa fluor 660 663 690 red Cy5.5 675 694 red TruRed 490 ... cory griffin staefarm inscory grinderWebAug 30, 2024 · Actin was stained with Texas-Red-phalloidin (red). Insets magnify the indicated area. Scale bar is 10 μm. (F) Fluorescence microscopy of cells expressing Emerald-vinculin (green) and Apple-actin (purple). Cells were infected with WT Salmonella, and still images taken from a live movie at 0, 6, 13, and 19 minutes post infection. Scale … bread and butter squash picklesWebMar 17, 2024 · Cells were counterstained with phalloidin-conjugated FITC or Texas Red. Nuclei were stained with DAPI. Cells were then washed twice as described above and observed under the fluorescence microscope. Isotypes and non-probed cells were used as controls. 4.8. Establishment of Gemcitabine-Resistant Pancreatic Cancer Cells. cory grom ameripriseWebTexas Red (and Texas Red-X) dye products. We offer a broad range of Texas Red and Texas Red-X dye products including secondary antibodies, labeling kits, bioconjugates, and … cory gromanWebTexas Red hydrazide is a derivative of Texas Red sulfonyl chloride made by reaction with hydrazine (Invitrogen). The result is a sulfonyl hydrazine group on the No. 5 carbon … bread and butter strain