WebFor tandem conjugates incorporating PerCP (e.g., PerCP-Cy5.5), the excitation and emission properties of PerCP and the kinetics of energy exchange between … WebCommonly used tandem fluorochromes used for flow cytometry such as PerCP-Cy5.5, PerCP-eFluor710, PE-Cy7, APC-Cy7, etc. vary in their ability to transfer energy from donor dye to acceptor dye across antibody lots and over time due to fluorescence resonance energy transfer differences.
Fluorochrome Options for Antibody Conjugation
WebFor example, two of the most common fluorochromes, FITC (blue) and PE (orange) have significant overlap. With conventional flow cytometers the overlapping signal is subtracted decreasing the overall detection efficiency, With antigens expressed at a high density such as CD4 this is not a problem. Web♦ CY 7 has a fluorescence emission maximum around 767nm. PerCP-CY 5.5 490nm max. absorption 695nm max. emission Argon Ion LASER (488nm) ♦ PerCP-CY 5.5 is a tandem conjugate system, combining PerCP and a cyanine dye. ♦ Excitation of CY 5.5 is about 675 (same as PerCP emission) and has a fluorescence emission maximum around 695nm events near me march 31 2023
What Is A Perc Test? 14 Things You Should Know in 2024
WebPerCP Peridinin-Chlorophyll-Protein (PerCP) is a red-emitting fluorescent protein isolated from algae that can be excited by the 488 nm blue laser and captured with a 670/30 nm … WebPerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It … WebInvitrogen Alexa Fluor 700 is a dim near-IR dye that can be excited with 633–640 nm lasers. Highly expressed antigens can be labeled with Alexa Fluor 700 in flow cytometry panels allowing for brighter dyes to be used with less-abundant antigens. It fits in a panel between APC and APC-eFluor 780. events near me long beach