WebFoxO1 Antibody detects endogenous levels of FoxO1 protein and FoxO4 (at 65-70 kDa). The antibody is sensitive to phosphorylation within the antigenic site and will … WebDetection of human FOXO1a by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer.Antibodies: Affinity purified rabbit anti-FOXO1a antibody A300-297A (lot A300-297A-3) used for IP at 6 µg per reaction. FOXO1a was also immunoprecipitated by …
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WebJul 1, 2010 · Reactivation of FOXO1A by stable or transient transfection also restored the growth-inhibitory effects of trastuzumab in SKBR3/AA28, BT474/AA9, and MCF7-HER2 cells. Knocking down FOXO1A by small interfering RNA resulted in reducing trastuzumab-induced growth inhibition. ... Antibodies, Monoclonal / pharmacology* Antibodies, … WebFOXO1 is involved in the insulin regulation of glucose-6-phosphatase (G6p) expression. Additionally, this protein plays a vital role in tumor suppression by inducing growth arrest and apoptosis. Features and Benefits Evaluate our antibodies with complete peace of mind.
WebMar 21, 2024 · GeneCards Summary for FOXO1 Gene. FOXO1 (Forkhead Box O1) is a Protein Coding gene. Diseases associated with FOXO1 include Rhabdomyosarcoma 2 … WebFKHR Antibody (C-9) is an IgG 1 κ mouse monoclonal FKHR antibody (also designated FOXO1A antibody) that detects the FKHR protein of mouse, rat and human origin by …
WebAnti-FOXO1 antibodies are offered by a number of suppliers. This target gene encodes the protein 'forkhead box O1' in humans and may also be known as FKHR, FKH1, FOXO1A, forkhead box protein O1, and forkhead box protein O1A. Structurally, the protein is reported to be 69.7 kilodaltons in mass. WebJan 1, 2004 · D4476 effectively inhibited the phosphorylation of FOXO1a at Ser322 and Ser325, but not the PKB-catalysed phosphorylation of Thr24 , Ser256 and Ser319 (data not shown). The pSer325 antibody was consistently more sensitive to D4476 than the pSer322 antibody, with inhibition occurring at 0.2 and 2 μM, respectively.
WebPurified anti-FOXO1 Antibody. Whole cell extracts (15 µg total protein) from HEL, MOLT4 (negative controls), Hela, Daudi (positive controls) and EL4 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-FOXO1 antibody (clone W20064D) overnight at 4 °C.
WebAnti-FOXO1A antibody (ab39670) at 1/1000 dilution + Whole cell lysates prepared from human Jurkat cells at 200000 cells Secondary HRP-conjugated donkey polyclonal to rabbit IgG at 1/2000 dilution Developed using the ECL technique. Predicted band size: … boosted defineWebWhat buffer format is "FOXO1A Antibody (Phospho-Ser329) (OAAF07382)" provided in? This item is provided in "Liquid (without Mg 2+ and Ca 2+) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol ". Additional format options may be available. For more information please contact [email protected]. has the time change been eliminatedWebDec 30, 2010 · MCF-7cells treated FoxO1a,FoxO3a, FoxO4transcription factors (Fig. 1E) markedlyincreased controlcells (Fig. 1A GSK3a/hphosphorylation occurred bothDU-145 MCF-7cells beganafter 40 minutes rapamycinexposure, remaining elevated least24 hours after initiation rapamycintreatment. boosted discordWebBioss Anti-FOXO1A Polyclonal, Catalog # BS-9439R-PE. Tested in Flow Cytometry (Flow) applications. This antibody reacts with Bovine, Canine, Chicken, Human, Mouse, … boosteddesigbs discountWebSynthetic peptide corresponding to Human FOXO1A. Synthetic non-phosphopeptide derived from human FOXO1A around the phosphorylation site of serine 319.Database link: Q12778. Validations. None provided. Applications. Chromatin immunoprecipitation (ChIP) Enzyme-linked immunosorbent assay (ELISA) Immunocytochemistry-immunofluorescence (ICC-IF) boosted decision tree modelWebFoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free (NBP2-80741): Novus Biologicals View Mouse Monoclonal anti-FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free (NBP2-80741). Validated Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready. Validated Species: Human, Mouse, Rat. Skip to main content boosted discount codeWebFOXO1A Antibody (Phospho-Ser329) : Biotin (OAAF07382-Biotin), Catalog OAAF07382-Biotin. Tested in WB, IHC, ELISA applications. Reacts with Human, Mouse, Rat samples. Liquid. Purified antibody supplied in 1x PBS buffer. boosted drops with bbs ark